DNA methylation analysis

The best studied epigenetic modification is the methylation of DNA at cytosine bases within CpG dinucleotides (i.e. C followed by G). DNA methylation is essential to mammalian development and is a key factor in the regulation of gene expression. Depending on our client demands, we offer the following services:

Targeted Bisulfite Sequencing

 

Targeted Sanger Bisulfite Sequencing allows researchers to analyse gene regions of interest through cloning of bisulfite converted DNA fragment followed by sequencing of 10-15 clones. Amplicons are 250-450 bp in length.

Our Service includes:

-         Design of primers for the desired gene region to analyse.

-         Bisulfite conversion

-         Targeted Amplification

-         Cloning and Sequencing

-         Sequence analysis

-         Determination of DNA methylation

-         Reporting

Methylation-specific PCR

 

Methylation-specific PCR (MSP) permits gene/sequence-specific detection of DNA methylation. Bisulfite conversion of genomic DNA followed by amplification of methylated sequences with specific primers allows the detection of methylation at specific methylation sites within genes or genomic regions

Our Service includes:

-         Design of primers for the desired gene region to analyse.

-         Bisulfite conversion

-         Targeted Amplification

-         PCR Data Analysis and determination of DNA methylation levels

-         Reporting

 

5-mC DNA ELISA assay

The 5-mC DNA ELISA assay allows the quantitative analysis of global DNA methylation levels in a wide range of DNA samples. In addition, our workflow allows the analysis of a high number of samples in a short time.

 

Please contact us!